Descriptive Analysis Summary

Global Information

Performed Contrasts Alias Comparison
IF comparison AUD vs. Control in Females
IM comparison AUD vs. Control in Males
IS comparison (AUD vs. Control in Females) vs. (AUD vs. Control in Males)
Number of lipids Ion mode N
Negative 311
Positive 264
Total 575

Sample Class Summary

  • Organism:
    Human (Homo sapiens)

  • Number of samples per experimental group:
    • AUD Control Total
    Female Male Female Male
    5 6 6 6 23

Differential Abundance Summary

IF comparison IM comparison IS comparison Total Lipids
Significant LFC < 0 LFC > 0 Significant LFC < 0 LFC > 0 Significant LFC < 0 LFC > 0
32 21 11 39 23 16 15 8 7 575

Significant lipids are separated according to the sign of their log fold-change (LFC).
In IF and IM comparisons, LFC < 0 columns = Differential abundance lipids decreased in AUD condition; LFC > 0 columns = Differential abundance lipids increased in AUD condition.
In IS, LFC < 0 columns = Differential abundance lipids increased in males; LFC > 0 columns = Differential abundance lipids increased in females.

Lipid Set Analysis Summary

IF comparison IM comparison IS comparison
Significant LOR < 0 LOR > 0 Significant LOR < 0 LOR > 0 Significant LOR < 0 LOR > 0
1 1 0 1 0 1 3 2 1

Significant lipid classes are separated according to the sign of their log odds ratio (LOR).
In IF and IM comparisons, LOR < 0 columns = This class is enriched in control conditions; LOR > 0 columns = This class is enriched in ethanol conditions.
In IS, LOR < 0 columns = This class is increased in males; LOR > 0 columns = This class is increased in females.

Metabolic lipid network

The dysregulated subnetworks that maximize the reaction difference between the AUD and control conditions in females and males generated by LINEX2:



The resulting subnetworks include only PC and LPC lipid species in females, and Cer and SM in males, suggesting that the enzymatic dysregulations are involved in different types of biochemical reaction in both sexes, transforming the lipid species into each other.