[[pcamasigpro]]
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pcamasigpro [2010/03/12 16:47] mjnueda |
pcamasigpro [2014/05/12 12:42] (current) jcarbonell |
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| __Parameters for //PCA-maSigFun gene selection//__: | __Parameters for //PCA-maSigFun gene selection//__: | ||
| *//Data//: txt file with expression data, genes in rows, arrays in columns. The file must contain an additional row with arrays names and a column with gene names.\\ | *//Data//: txt file with expression data, genes in rows, arrays in columns. The file must contain an additional row with arrays names and a column with gene names.\\ | ||
| + | \\ | ||
| + | |Name|Array1|Array2|Array3|Array4|Array5|Array6|Array7|Array8|Array9|…| | ||
| + | |gene1|0.5|0.2|0.7|1.3|1.4|1.0|2.1|2.4|2.6|…| | ||
| + | |gene2|0.5|0.3|0.4|0.3|0.4|0.1|0.1|0.4|0.5|…| | ||
| + | |...|...|...|...|...|...|...|...|...|...|…| | ||
| + | \\ | ||
| *//Covariates//: txt file with experimental design information, containing as many columns as arrays and as many rows as experimental factor. Each cell contains the value of the array in the experimental factor. E.g: | *//Covariates//: txt file with experimental design information, containing as many columns as arrays and as many rows as experimental factor. Each cell contains the value of the array in the experimental factor. E.g: | ||
| \\ | \\ | ||
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| * Proportion of acumulated variability. Posible cut-off values are in (0,1). | * Proportion of acumulated variability. Posible cut-off values are in (0,1). | ||
| * Proportion of variability of each PC. Posible cut-off values are in (0,1). | * Proportion of variability of each PC. Posible cut-off values are in (0,1). | ||
| - | * Average: components are selected that explain more than "cut-off" times the average component variability, calculated as total data variability divided by the rank of the matrix associated to the factor. | + | * Average: components are selected that explain more than "cut-off" times the average component variability. The recommended "cut-off" values are in [1,1.5]. |
| \\ | \\ | ||
| __Parameters for //PCA-maSigFun visualization//__\\ | __Parameters for //PCA-maSigFun visualization//__\\ | ||
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| by applying several procedures: | by applying several procedures: | ||
| *//Resampling//: where a null Leverage distribution is created by permuting columns of expression data and genes are selected at the "alpha" percentile of the null distribution. | *//Resampling//: where a null Leverage distribution is created by permuting columns of expression data and genes are selected at the "alpha" percentile of the null distribution. | ||
| - | *//minAS//: where a density function is calculated on the data and genes are selected on a local minimum basis. | + | *//minAS//: where a density function is calculated on the data and genes are selected on a local minimum basis [[references |[7]]]. |
| - | *//Gamma//: where a gamma distribution is adjusted to the distributions of the gene loadings, and genes are selected at the "alpha" percentile of the gamma distribution. | + | *//Gamma//: where a gamma distribution is adjusted to the distributions of the gene loadings, and genes are selected at the "alpha" percentile of the gamma distribution [[references |[7]]]. |
| *//Custom//: where the user can decide the threshold. | *//Custom//: where the user can decide the threshold. | ||
| \\ | \\ | ||
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