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SEA accepts three types of data files: expression, covariates and annotation. Data files are always tab-delimited files. strange symbols (such as % , &, /, # , etc) and quotation marks (“”) should be avoided in labels and gene names. Also the presence of spaces is not recommended.
We next explain each data file in detail:
* Expression data: txt file with expression data, genes in rows, arrays in columns. The file must contain an additional row with sample names and a column with gene names:
gene_names | sample_1 | sample_2 | sample_3 | sample_4 | sample_5 | sample_6 | sample_7 | sample_8 | sample_9 |
gene_1 | 0.18 | 0.53 | 0.43 | -0.99 | -2.45 | -1.18 | 1.43 | 2.32 | 0.32 |
gene_2 | 1.22 | 0.91 | 1.52 | 1.23 | -0.33 | 1.58 | -2.31 | -1.27 | -1.08 |
|Time|3|3|3|9|9|9|27|27|27|…|
Treatment | Ctr | TrA | TrB | Ctr | TrA | TrB | Ctr | TrA | TrB | … |
Time | 3 | 3 | 3 | 9 | 9 | 9 | 27 | 27 | 27 | … |
Treatment | Ctr | TrA | TrB | Ctr | TrA | TrB | Ctr | TrA | TrB | … |
Time | 3 | 3 | 3 | 9 | 9 | 9 | 27 | 27 | 27 | … |
Treatment | Ctr | TrA | TrB | Ctr | TrA | TrB | Ctr | TrA | TrB | … |