DATA

We have selected the first 200 genes of a breast cancer study with healthy and patient tissues. We want to know if there is any kind of structure in this dataset.

Normalized expression set: brca_tmm_normalized_results_sel200.txt

EXERCISE

1. Go to Expression → Unsupervised Analysis Clustering
2. Select the expression set
3. Select clustering of samples AND genes
4. Left all other parameters as default
5. Launch job

QUESTIONS

1. Is there a structure in the data
2. The heatmap in the Differential expression tool also performs an unsupervised clustering. Which differences do you find?
3. Why do you think the heatmap is showing more clear differences than this clustering?