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result_interpretation [2012/05/03 15:32]
mbleda [Interpretation]
result_interpretation [2012/05/03 15:33] (current)
mbleda [Interpretation]
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 When significant results are obtained, we can suppose that there is one or several regulatory elements behaving different when comparing groups. The list of genes included in the analysis have pointed to a significantly over-represented set of common regulators to these genes. The interpretation of the results will be different in the case of TFs (transcription factors) and miRNAs given that (generally) the first are positive regulators and the latter are negative regulators. ​ When significant results are obtained, we can suppose that there is one or several regulatory elements behaving different when comparing groups. The list of genes included in the analysis have pointed to a significantly over-represented set of common regulators to these genes. The interpretation of the results will be different in the case of TFs (transcription factors) and miRNAs given that (generally) the first are positive regulators and the latter are negative regulators. ​
  
-**TFs** generally bind to the promoter region of their target genes to assist and promote the transcription. ​**miRNAs**, on the other hand, bind to transcript products preventing them from being translated. Significant TF and miRNAs can be pointed to be responsible for the differential expression of the genes observed in the list. We must take special care in the interpretation of over-expressed or under-expressed genes in a functional analysis. In the case of TFs, if we are working with the list of over-expressed genes, the significant results makes reference to active TFs in one condition with respect to the other; while significant results of under-expressed genes makes reference to inactive TFs. In miRNAs, significant results of over-expressed genes will point to inactive miRNAs, while significant results of under-expressed genes will point to active miRNAs when comparing conditions. The following table summarizes the result interpretation:​+TFs generally bind to the promoter region of their target genes to assist and promote the transcription. miRNAs, on the other hand, bind to transcript products preventing them from being translated. Significant TF and miRNAs can be pointed to be responsible for the differential expression of the genes observed in the list. We must take special care in the interpretation of over-expressed or under-expressed genes in a functional analysis. In the case of **TFs**, if we are working with the list of over-expressed genes, the significant results makes reference to active TFs in one condition with respect to the other; while significant results of under-expressed genes makes reference to inactive TFs. In **miRNAs**, significant results of over-expressed genes will point to inactive miRNAs, while significant results of under-expressed genes will point to active miRNAs when comparing conditions. The following table summarizes the result interpretation:​
  
 ^                        ^  TFs                      ^  miRNAs ​ ^ ^                        ^  TFs                      ^  miRNAs ​ ^
result_interpretation.txt ยท Last modified: 2012/05/03 15:33 by mbleda
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