[[valprog]]

PAST COURSES

Program Valencia Edition

Monday 28th September, Functional Annotation with Blast2GO

9:00-10:30 Session 1

  • Concepts of functional Annotation:
    • Homology based function transfer
    • The Gene Ontology Resource
    • The Blast2GO Annotation Rule

10:30-11:00 Coffee break
11:00-13:00 Session 2

  • The Blast2GO annotation framework:
    • BLAST, Annotate, Analyze
    • Diagnosis charts
    • Data selection, import/export

13:00-14:00 LUNCH

14:00-15:30 Session 3

  • Functional Annotation Strategies

15:30-16:00 Coffee break
16:00-17:30 Practical Session 1

  • Hands-On: Annotation
    • You will annotate an example dataset, learn how to generate GO, InterPro and KEGG annotations, modulate intensity of annotation, curate annotation data and export results in different formats
    • Bring and annotate your own data!!!

Tuesday 29th September. Data-mining on Functional Annotation

9:00-10:30 Session 4

  • Summarizing functional Information:
    • Exploring function on the Gene Ontology DAG
    • Analysis of functional distributions, optimization strategies

10:30-11:00 Coffee break
11:00-13:00 Session 5

  • Analysis of functional differences (enrichment methods)
    • Fisher's Exact Test (Gossip (B2G), Fatigo (Babelomics))
    • Gene Set Enrichment Analysis (Fatiscan (Babelomics))
  • Analysis of functional similarities (GoetzScore)

13:00-14:00 LUNCH

14:00-15:30 Session 6

  • Behind B2G: Technical issues
    • Set up a local B2G database
    • How to set up a local Blast server
    • B2G fine-tuning: The B2G-Property file
    • Advanced options

15:30-16:00 Coffee break
16:00-17:30 Practical Session 2

  • Hands-On: Functional Analysis
    • Students will work out examples of functional queries with Blast2GO
    • Bring and analyze your own data!!!

Wednesday 30th September. Functional Annotation Workshop: Use cases

9:00-10:30

  • Knut Erik Tollefsen.Development and evaluation of a mussel (Mytilus sp.) oligonucleotide microarray.
  • Harrold A van den Brug.Studying plant diseases by comparative genomics between fungal plant pathogens.
  • Björn Rotter.SuperSAGE and normalized cDNA sequencing for the analysis of non-model organisms
  • Neil Bower. Identification of novel genes involved in muscle growth in Atlantic salmon

10:30-11:00 Coffee break
11:00-12:00

  • Gareth Pearson.Gene expression during heat shock in the intertidal seagrass Zostera noltii.
  • Saneyoshi Ueno. Transcripts from Quercus petraea winter buds - 454 pyrosequencing.
  • Juan José González. Generation of bioinformatics tools for the study of olive tree.
  • Céline Muys. Industrial chicory EST dataset generation and analysis

12:00-13:00 Discussion

13:00-14:00 LUNCH

14:00-14:30

  • Concluding remarks and course closure
valprog.txt · Last modified: 2009/10/18 22:19 by aconesa
Bioinformatics and Genomics Department
Centro de Investigación Príncipe Felipe
Valencia, SPAIN
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