9:00-10:30 Session 1

• Concepts of functional Annotation:
  • Homology based function transfer
  • The Gene Ontology Resource
  • The Blast2GO Annotation Rule

10:30-11:00 Coffee break
11:00-13:00 Session 2

• The Blast2GO annotation framework:
  • BLAST, Annotate, Analyze
  • Diagnosis charts
  • Data selection, import/export

13:00-14:00 LUNCH

14:00-15:30 Session 3

• Functional Annotation Strategies

15:30-16:00 Coffee break
16:00-17:30 Practical Session 1

• Hands-On: Annotation
  • You will annotate an example dataset, learn how to generate GO, InterPro and KEGG annotations, modulate intensity of annotation, curate annotation data and export results in different formats
  • Bring and annotate your own data!!!

9:00-10:30 Session 4

• Summarizing functional Information:
  • Exploring function on the Gene Ontology DAG
  • Analysis of functional distributions, optimization strategies

10:30-11:00 Coffee break
11:00-13:00 Session 5

• Analysis of functional differences (enrichment methods)
  • Fisher's Exact Test (Gossip (B2G), Fatigo (Babelomics))
  • Gene Set Enrichment Analysis (Fatiscan (Babelomics))
• Analysis of functional similarities (GoetzScore)

13:00-14:00 LUNCH

14:00-15:30 Session 6

• Behind B2G: Technical issues
  • Set up a local B2G database
  • How to set up a local Blast server
  • B2G fine-tuning: The B2G-Property file
  • Advanced options

15:30-16:00 Coffee break
16:00-17:30 Practical Session 2

• Hands-On: Functional Analysis
  • Students will work out examples of functional queries with Blast2GO
  • Bring and analyze your own data!!!

9:00-10:30

• Knut Erik Tollefsen.Development and evaluation of a mussel (Mytilus sp.) oligonucleotide microarray.
• Harrold A van den Brug.Studying plant diseases by comparative genomics between fungal plant pathogens.
• Björn Rotter.SuperSAGE and normalized cDNA sequencing for the analysis of non-model organisms
• Neil Bower. Identification of novel genes involved in muscle growth in Atlantic salmon

10:30-11:00 Coffee break
11:00-12:00

• Gareth Pearson.Gene expression during heat shock in the intertidal seagrass Zostera noltii.
• Saneyoshi Ueno. Transcripts from Quercus petraea winter buds - 454 pyrosequencing.
• Juan José González. Generation of bioinformatics tools for the study of olive tree.
• Céline Muys. Industrial chicory EST dataset generation and analysis

12:00-13:00 Discussion

13:00-14:00 LUNCH

14:00-14:30

• Concluding remarks and course closure