[[saprog]]

Workshop Outline Cape Town

Thursday Dec. 03

Functional Annotation

  • 08h30-09h00 Registration & Welcome
  • 09h00-11h00 Functional Annotation
    • Concepts of functional Annotation
    • Homology based function transfer
    • The Gene Ontology Resource
  • 11h00-11h30 Tea/Coffee break
  • 11h30-13h00 Introduction to Blast2GO
    • The Blast2GO annotation framework: BLAST, Annotate, Analyze
    • Learn how to create functional labels with Blast2GO
  • 13h00-14h00 LUNCH BREAK
  • 14h00-14h45 Annotation Strategies
    • Learn how to adjust B2G parameters depending on your objectives and data
  • 14h45-15h30 Behind B2G
    • Set up a local B2G database
    • How to set up a local BLAST server
    • B2G fine-tuning: All about the Blast2GO property file
  • 15h30-16h00 Tea/Coffee break
  • 16h00-18h00 Hands-On: Annotation (2h approx.)
    • Annotate of an example (or user) dataset:
    • Learn how to generate GO, InterPro and KEGG annotations
    • Modulate intensity of annotation
    • Curate annotation data and export results in different formats
  • 19h00 DINNER

Friday Dec. 04

Data-mining on Functional Annotation

  • 09h00-11h00 Summarizing functional Information:
    • Exploring function on the Gene Ontology DAG
    • Analysis of functional distributions, optimization strategies
  • 11h00-11h30 Tea/Coffee break
  • 11h30-13h00 Functional Enrichment/Similarity
    • Analysis of functional differences (enrichment methods)
    • Fisher's Exact Test (Gossip (B2G), Fatigo (Babelomics))
    • Gene Set Enrichment Analysis (Fatiscan (Babelomics))
    • Analysis of functional similarities (GoetzScore)
  • 13h00-14h00 LUNCH BREAK
  • 14h00-15h30 Hands-On: Functional Analysis
    • Students will work out examples of functional queries with Blast2GO
    • Students have the possibility to analyze own datasets
  • 15h30-16h00 Tea/Coffee break
  • 16h00-17h30 Workshop Round Table:
    • Time for discussions, questions, feedback and wishes.
    • Participants are also most welcome to present their functional annotation/analysis projects or results.
saprog.txt · Last modified: 2009/12/02 13:20 by aconesa
Bioinformatics and Genomics Department
Centro de Investigación Príncipe Felipe
Valencia, SPAIN
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