• Arrival and registration

7:30-8:30: Continental Breakfast
8:30-10:00: Concepts of functional Annotation

• Homology based function transfer
  
• Functional Annotation Schemes
  
• The Gene Ontology Resource
  
• The Blast2GO Annotation Rule
  

10:00-10:30: Coffee
10:30-12:30: The Blast2GO annotation framework:

• BLAST, Annotate, Analyze
  
• Diagnosis charts
  
• Functional Annotation Strategies and Customization
  

12:30-13:30: LUNCH provided by Hotel
13:30-15:30: Functional Annotation Strategies

• Learn about the different annotation parameters and annotation strategies and its impact on the generated annotation

15:30-16:00: Coffee
16:00-17:30: Hands-On: Annotation

• You will annotate an example dataset, learn how to generate GO, InterPro and KEGG annotations, modulate intensity of annotation, curate annotation data and export results in different formats
• Bring and annotate your own data!!!

7:30-8:30: Continental Breakfast
8:30-10:00: Summarizing functional Information

• Exploring function on the Gene Ontology DAG
• Analysis of functional distributions, optimization strategies

10:00-10:30: Coffee
10:30-12:30: Exercises: Quantitative Analysis:

• Perform customized graphs and pie-charts
• Filter data depending on functional characteristics

12:30-13:30: LUNCH provided by Hotel
13:30-15:30: Statistical assessment of functional differences:

• Analysis of functional differences (enrichment methods)
• Fisher's Exact Test (Gossip (B2G), Fatigo (Babelomics))
• Gene Set Enrichment Analysis (Fatiscan (Babelomics))
• Analysis of functional similarities (GoetzScore)

15:30-16:00: Coffee
16:00-17:30: Exercises: Functional Profiling

• Analyse various dataset to detect functional differences and/or similarities
  

7:30-8:30: Continental Breakfast
8:30-10:00: Behind B2G

• Set up a local B2G database
• How to set up a local Blast server
• B2G fine-tuning: The B2G-Property file
• Advanced options

10:00-10:30: Coffee
10:30-12:30: Workshop

• Abdelali Barakat. The Penn State University.Analyses of the transcriptome of the Fagaceae species
• Ana Maria Bocsanczy. University of Florida. Annotation of apple (malus) ESTs from an oligo microarray
• Matthew Riley . Walter Reed Army Institute of Research. Functional Annotation in Malaria
• Brenda Oppert USDA ARS Grain Marketing and Production Research Center.The Trials and Tribulations of Blast2Go and NextGen Sequencing.
• Richard Westerman Prude University. Pipelining 454 sequencing into protein contigs with subsequent annotation via blast2go
• Ross Whetten North Carolina State University. Analysis of sequence data from a population sample of Fraser fir.

12:30-13:30: LUNCH provided by Hotel
13:30-14:00: Concluding remarks and course closure